Gene Annotation

Eureka Genomics provides detailed gene annotation services to identify structural and functional elements of whole and partial genome sequences. Gene annotation can be performed on contigs assembled from sequences from any species and sample type including human mRNA. The annotation process includes extensive BLAST searches for gene prediction (ORFs) and searches against protein sequence databases for gene function prediction. The sequences to be submitted for annotation can be generated using Eureka Genomics' Next Generation Sequencing (NGS) Services. Unassembled sequence data generated elsewhere using Illumina's Genome Analyzer is accepted. Unassembled sequences generated on Roche 454 and ABI SOLiD instruments may also be considered; please contact Eureka Genomics to discuss options.

The deliverables of Eureka Genomics' Standard Annotation Service are three excel spreadsheets. Click on each table heading to see an example of the deliverables.

• Table 1. ORF Prediction



-Contig ID	-Strand
-orfID	-Nucleotide Length
-ORF start	-Gene Symbol
-ORF end	-Gene Name/Description
-Frame	-COG (when applicable)

• Table 2. Gene Annotation



-orfID	-Protein Length	-Ligand ID
-Gene Symbol	-Molecular Weight	-Ligand Description
-Gene Name	-Operon (when applicable)	-Tissue specific expression (when applicable)
-Protein Name	-Isoelectric point	-Disease involvement (when applicable)
-Gene Description	-Protein Domain Accession #	-pH (when applicable)
-Protein Symbol	-Protein Domain Description	-Phenotype
-Protein Ontology	-Ligand Interaction	-Mutation (when applicable)
-Protein Function	-Gene Expression Regulation	-Special Characteristics-Nitrogen, pH, Carbohydrates
-Kegg Pathway ID	-Gene Expression Classification	(when applicable)
-Kegg Pathway Description	-Gene Expression Condition
-GOID	-Drug Response Classification
-Gene Ontology Text	-Drug Response Regulation
-Localization	-Drug Response Condition

• Table 3. Codon Usage



-Triplet Codon

-Frequency per thousand

-Number

In some organisms, completeness of annotation depends on extent of homology information in public databases.

Manual curation and custom annotation are available. Please contact Eureka Genomics to discuss.