Sample and Documentation Requirements for the submission of EACH LOW STARTING AMOUNT DNA sample

Use any conventional method or commercially available DNA isolation kits to extract DNA. Treat with RNase if necessary to rid RNA contamination from DNA sample. If more DNA is available than the quantity specified below, please use the DNA sequencing service. If the quality is poorer than specified below, please contact us to discuss alternatives. All DNA should be resuspended in nuclease-free water or 10mM Tris-HCl pH 8.5 buffer. Be sure to include Completed DNA Sample Submission Form with each sample submission.

1 - 50 ng DNA Sample
Sample Quality: A260/280 ≥1.8 For low DNA concentrations, analysis with the Qubit Spectrophotometer is preferred.

Sample Documentation: Provide printout of 260/280 result.

Sample Quantity: 1 - 50 ng ≥ 0.5ng/uL

51 - 699 ng DNA Sample
Sample Quality: A260/280 ≥1.8 along with gel run with high molecular weight ladder showing little to NO DNA sample degradation and RNA contamination. DNA should be mostly intact and of high molecular weight.

Sample Documentation: Provide printout of A260/280 and picture of gel with clearly labeled ladder and sample(s).

Sample Quantity: 51 - 699 ng at ≥ 10ng/uL

Sequencing Types Definitions:
SR = Single End Read
PE = Paired End Read
SR—MP = Single End Read, Multiplexed
PE—MP = Paired End Read, Multiplexed