Sample and Documentation Requirements for the submission of EACH DNA sample

Use any conventional method or commercially available DNA isolation kits to extract DNA. Treat with RNase if necessary to rid RNA contamination from DNA sample. If less DNA is available than the quantity specified below, please contact us to discuss alternatives or consider the 1 ng sequencing service. If the quality is poorer than specified below, please contact us to discuss alternatives. All DNA should be resuspended in nuclease-free water or 10mM Tris-HCl pH 8.5 buffer. Be sure to include Completed DNA Sample Submission Form with each sample submission.

Total DNA Sample
Sample Quality: A260/280 ≥1.8 and a gel run with high molecular weight ladder showing little to NO DNA sample degradation and RNA contamination. DNA should be mostly intact and of high molecular weight.

Sample Documentation: Provide printout of 260/280 result and picture of gel with clearly labeled ladder and sample(s).

Sample Quantity: 0.7-4 ug at ≥ 100ng/uL unless arranged otherwise on Service Agreement

Amplified DNA Sample
Sample Quality: A260/280 ≥1.8 along with gel showing defined band at the expected size of the amplicon(s). If the DNA is a product of whole genome amplification, a gel run with high molecular weight ladder showing little to NO DNA degradation and RNA contamination.

Sample Documentation: Provide printout of A260/280 and picture of gel with clearly labeled ladder and sample(s).

Sample Quantity: 0.7-4 ug at ≥ 100ng/uL unless arranged otherwise on Service Agreement.

Sequencing Types Definitions:
SR = Single End Read
PE = Paired End Read
SR—MP = Single End Read, Multiplexed
PE—MP = Paired End Read, Multiplexed