Custom Design of Ultra-Specific
Signatures for Pathogen Detection

The cornerstone of a molecular diagnostic assay is the specificity of the nucleic acid sequence that is detected. Eureka Genomics offers the in silico design of ultra-specific signature sequences based on full genome comparisons. These ultra-specific signatures will be designed to the requirements of your platform (microarray probes, PCR primers, RT-PCR probes/primers, etc.). Genetic information used in the design of ultra-specific signatures can come from the Eureka Genomics' data collection, be provided by the client, or be generated de novo by Eureka Genomics.

Eureka Genomics approach to Ultra-specific Signature Design is unique:

• Generates ultra-specific signature sequences that are 2, 3, or 4 mismatches (insertions, deletions and substitutions on any position and in any combination) away from all subsequences in the host/background genomic materials.
• Hundreds of complete genomes can be compared in one run.
• Design the minimal combination of the ultra-specific genomic signatures (ultra-specific assay) to detect and distinguish a specific genomic target from a known background (such as identify dengue virus in presence of human, mosquito, and bird genomic background and distinguish its serotypes). Design modified to your platform (microarray probes, PCR primers, RT-PCR probes/primers, etc.).
• If insufficient genomic sequence data is available, Eureka Genomics can sequence customer samples.
• Contact us for further information. Click here for further reading.