Custom Design of Ultra-Specific
Signatures for Pathogen Sub-Typing

The cornerstone of a molecular diagnostic assay is the specificity of the nucleic acid sequence that is detected. This is complicated when the subtypes of the pathogen need to be detected in a single assay. Based on full genome comparisons Eureka Genomics offers the in silico design of multiplexed ultra-specific signature sequences for pathogen subtyping. These ultra-specific signatures will be designed to the requirements of your platform (microarray probes, PCR primers, RT-PCR probes/primers, etc.). Genetic information used in the design of ultra-specific signatures can come from the Eureka Genomics' data collection, be provided by the client, or be generated de novo by Eureka Genomics.

Eureka Genomics approach to Ultra-Specific Signature Design is unique.

• Ultra-specific signature sequences that are 2, 3, or 4 mismatches (insertions, deletions and substitutions on any position and in any combination) away from all subsequences in the host/background genomic materials are generated.
• Hundreds of complete genomes and genes can be used in the background.
• The minimal combination of the ultra-specific genomic signatures (ultra-specific assay) to detect and distinguish any number of subtypes from a background (clinical sample) can be designed.
• The design is modified to your platform (array probes, multiplex PCR primers, multiplex RT-PCR probes/primers, etc.).
• If insufficient genomic sequence data is available, Eureka Genomics can sequence customer samples.
• Contact us for further information. Click here for further reading.