Analysis of the Quality of Sequencing Reads

Eureka Genomics performs Analysis of the Quality of Illumina Sequencing Reads. This allows quick assessment of the quality of the data and includes graphs presenting tile-by-tile, nucleotide-by-nucleotide and cycle-by-cycle distributions of cluster densities, -nucleotides frequencies, and quality scores. This information can be used to determine if some tiles should be removed from further analysis, and/or if trimming (cutoff) of sequencing reads should occur. This report can also be used for identification of various sources of sample contamination, such as unintentional sequencing of adapters or PCR primers used in sample preparation. Click here for an example anonymized Quality Report