The cornerstone of a molecular diagnostic assay is the specificity of the nucleic acid sequence that is detected. This is more complicated when the subtypes of the pathogen need to be detected in a single assay or when multiple pathogens need to be detected in a single assay. Eureka Genomics offers in silico design of ultra-specific signature sequences based on full genome comparisons. These ultra-specific signatures will be designed to the requirements of your platform (microarray probes, PCR primers, RT-PCR probes/primers, etc.). The ultra-specific signatures can be designed for single pathogen detection, multiplex pathogen detection or pathogen sub-typing. Genetic information used in the design of ultra-specific signatures can come from the Eureka Genomics' data collection, be provided by the client (as reference sequence or GenBank accession numbers), or be generated de novo by Eureka Genomics.

Eureka Genomics approach to Ultra-specific Signature Design is unique:

• Generates ultra-specific signature sequences that are 2, 3, or 4 mismatches (insertions, deletions and substitutions on any position and in any combination) away from all subsequences in the host/background genomic materials.
• Hundreds of complete genomes can be compared in one run.
• Design the minimal combination of the ultra-specific genomic signatures (ultra-specific assay) to detect and distinguish a specific genomic target from a known background (such as identify dengue virus in presence of human, mosquito, and bird genomic background and distinguish its serotypes). The signature sequences are optimized for your platform (microarray probes, PCR primers, RT-PCR probes/primers, etc.).
• The minimal combination of the ultra-specific genomic signatures (ultra-specific assay) to detect and distinguish any number of subtypes from a background (clinical sample) can be designed.
• The minimal combination of the ultra-specific genomic signatures (ultra-specific assay) to detect and distinguish any number of genomic targets from a background (clinical sample) can be designed.
• If insufficient genomic sequence data is available, Eureka Genomics can sequence customer samples.
• Signatures for hundreds of genomic targets can be designed in one run.