Library preparation

Eureka Genomics® can prepare DNA or RNA sequencing libraries for the purpose of next generation sequencing on any Illumina platform. The submitted DNA material can be isolated genomic DNA, cDNA or amplified DNA. TruSeq and Nextera libraries are available. TruSeq libraries can be prepared from low starting amount DNA samples, down to 1 ng/sample. The submitted RNA material can be total RNA, mRNA or microRNA. The prepared library can be returned to the Client or it can be sequenced at Eureka Genomics. Many sequencing and cycle choices are available. The library must be specifically prepared for the type of sequencing desired.
  1. A Single End Library allows sequence data to be generated from only one end of the library construct. A Single End library is useful when the paired end information is not specifically needed.
  2. A Paired End Library allows sequence data to be generated from both ends of the library constructs. The paired end information can be used to distinguish structural rearrangements such as insertions, deletions and inversions. Paired end information can be useful in finishing genome assemblies. A Paired End Library can also be processed for single end sequencing.
  3. A Mate-Pair Library allows sequence data to be generated from both ends of large DNA fragments (up to 5 kb). The Mate Pair information can be used to distinguish long-range structural rearrangements such as insertions, deletions and inversions, as well as to span long repeats during de novo assembly. This allows the generation of longer contigs and scaffolds and removes assembly ambiguities arising from the presence of long repeats in a genome
  4. For mRNAseq a Paired End Non Indexed Library or a Paired End Indexed Library can be generated from mRNA (Items 2 and 3 above). The starting material can either be high quality total RNA or mRNA.
  5. A Single End Non Indexed microRNA Library (Item 1 above) can be constructed from microRNA. The starting material can be either high quality Total RNA or pre-isolated microRNA.
All libraries can be prepared as Indexed Libraries for Multiplex sequencing. This is useful with samples that require a smaller amount of sequence data. Up to 12 samples (standard) can be uniquely tagged with index sequences and can be combined into a single lane of a flowcell for Multiplex Sequencing. During data analysis the individual samples in the lane can be parsed out based upon their unique index tags. If more than 12 barcodes are needed, contact us.
Eureka Genomics Corporation: 410 Pierce Street, Ste. 307, Houston, TX 77002 | 750 Alfred Nobel Drive, Hercules, CA 94547
Phone: (510) 964-0461 | Fax: (510) 964-9705 | Email: contact@eurekagenomics.com