Prepared Illumina Libraries

We recommend using Illumina supplied adaptors and oligos for preparing libraries. If less sample is available than the quantity specified below or if the quality is poorer than what is specified, please contact us to discuss alternatives. All Prepared Illumina Library Sample(s) should be re-suspended in Nuclease free water or 10mM Tris-HCl pH 8.5 buffer. Be sure to include a completed prepared library sample submission form with each sample submission.

DNA Prepared Library Sample

Illumina Sample quality: A single peak at ~300bp when analyzed on the DNA1000 chip of the Agilent Bioanalyzer 2100. There should be no other peaks except the markers at 15 and 1500bp. All 13 points of the ladder should be distinctly identifiable. A gel picture of the library with a well-defined ladder will be accepted if a Bioanalyzer is not available. Please include quantification results.

Illumina Sample documentation: Provide PDF printout of Agilent Bioanalyzer 2100 results of the final Prepared Library(ies). Provide sequences of adaptors and PCR primers and index primer(s) used (if intended for multiplex sequencing) on the Prepared Library Sample Submission Form. The Prepared Library(ies) must be compatible with sequencing primers and flowcell provided by Illumina. For Paired End Library(ies), DNA insert only size must be stated on the Prepared Library Sample Submission Form.

Sample quantity: 30 uL at ≥ 10nM unless arranged otherwise on Service Agreement.

mRNASeq Prepared Library Sample

Sample quality: A single peak between ~200bp and ~300bp when analyzed on the DNA1000 chip of the Agilent Bioanalyzer 2100. There should be no other peaks except markers at 15bp and 1500bp. All 13 points of the ladder should be distinctly identifiable. A gel picture of the library with a well-defined ladder will be accepted if a Bioanalyzer is not available. Please include quantification results.

Sample documentation: Provide PDF printout of Agilent Bioanalyzer 2100 results of the final Prepared Library(ies). Provide sequences of adaptors and PCR primers and index primer(s) used (if intended for multiplex sequencing) on the Prepared Library Sample Submission Form. The Prepared Library(ies) must be compatible with sequencing primers and flowcell provided by Illumina. For Paired End Library(s), cDNA insert only size must be stated on the Prepared Library Sample Submission Form.

Sample quantity: 25 uL at ≥ 10nM unless arranged otherwise on Service Agreement

microRNA Prepared Library Sample

Sample quality: Either a single product or two products between ~140bp and ~1650 bp when analyzed on the DNA1000 chip of the Agilent Bioanalyzer 2100. There should be no other peaks except markers at 15 and 1500bp. All 13 points of the ladder should be distinctly identifiable.

Sample documentation: Provide PDF printout of Agilent Bioanalyzer 2100 results of the final Prepared Library(ies). Provide sequences of adaptors and PCR primers and index primer(s) used (if intended for multiplex sequencing) on the Prepared Library Sample Submission Form. The Prepared Library(ies) must be compatible with sequencing primers and flowcell provided by Illumina.

Sample quantity: 10 uL at ≥ 10nM unless arranged otherwise on Service Agreement.

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